Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Moeller, H.; Boehrsch, V.; Lucka, L.; Hackenberger, C.P.; Hinderlich, S.
    Efficient metabolic oligosaccharide engineering of glycoproteins by UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE) knock-down (2011), Mol. Biosyst., 7, 2245-2251.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.60 molecular biology GNE-deficient cells, with dramatically increased incorporation of N-acetylmannosamine analogues into glycoproteins, can efficiently be decorated with reactive functional groups, which can be employed in bioorthogonal functionalization strategies for fluorescence labelling or biotinylation Homo sapiens
3.2.1.183 biotechnology a GNE-deficient HEK293 cell line proves its potential for the production of glycoproteins with modified sialylation, gaining therapeutic and diagnostic glycoproteins, and efficient application of metabolic oligosaccharide engineering Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.60 expression of GFP-tagged enzyme in HEK-293 cells, the expression is efficiently blocked by sh70-shRNA, sh68 has no effect on GNE protein expression because its target sequence at the 3'-UTR is not present in the GFP-hGNE vector construct Homo sapiens
3.2.1.183 gene GNE Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.60 additional information stable knock-down of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase in HEK-293 cells by RNAi dramatically increases incorporation of N-acetylmannosamine analogues into glycoproteins of HEK-293 cells. HEK293 cells transfected with sh68- and sh70-shRNA almost completely loose GNE mRNA expression, while other shRNAs are less effective. Peanut agglutinin, recognizing asialo structures, reveals a 3-4fold higher binding affinity to GNE knock-down cells compared to controls Homo sapiens
3.2.1.183 additional information efficient metabolic oligosaccharide engineering of glycoproteins by GNE enzyme knockdown by specific shRNAs in HEK293 cells. Stable GNE knockout via RNAi dramatically increases incorporation of N-acetylmannosamine analogues into glycoproteins of HEK293 cells. By means of these GNE-deficient cells highly sialylated glycoproteins can efficiently be decorated with reactive functional groups, which can be employed in bioorthogonal functionalization strategies for fluorescence labelling or biotinylation Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.183 UDP-N-acetyl-alpha-D-glucosamine + H2O Homo sapiens
-
 N-acetyl-D-mannosamine + UDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.60 Homo sapiens
-
-
-
3.2.1.183 Homo sapiens
-
-
-
5.1.3.14 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.183 HEK-293 cell
-
 Homo sapiens
-
5.1.3.14 HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.183 UDP-N-acetyl-alpha-D-glucosamine + H2O
-
 Homo sapiens N-acetyl-D-mannosamine + UDP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.60 GNE
-
 Homo sapiens
2.7.1.60 UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
-
 Homo sapiens
3.2.1.183 GNE
-
 Homo sapiens
3.2.1.183 UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
-
 Homo sapiens
5.1.3.14 GNE
-
 Homo sapiens
5.1.3.14 UDP-GlcNAc 2-epimerase/ManNAc kinase
-
 Homo sapiens
5.1.3.14 UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.1.60 malfunction stable knock-down of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase in HEK-293 cells dramatically increases incorporation of N-acetylmannosamine analogues into glycoproteins of HEK-293 cells Homo sapiens
2.7.1.60 metabolism UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase is the key enzyme in the sialic acid biosynthetic pathway Homo sapiens
3.2.1.183 metabolism key enzyme in the sialic acid biosynthetic pathway Homo sapiens
5.1.3.14 malfunction stable knock-down of GNE dramatically increases incorporation of N-acetylmannosamine analogues into glycoproteins of HEK-293 cells Homo sapiens
5.1.3.14 metabolism GNE is the key enzyme in the sialic acid biosynthetic pathway Homo sapiens